To help diabetic patients, human insulin has been mass produced by genetically engineering bacteria for many years. Genetic engineering may be controversial, yet it has its benefits. The thought of being able to genetically transform an organism is exciting yet intriguing at the same time. Come and immerse yourself in the magnificent world of genetic engineering and genetically transform bacteria to glow green (fluoresce)!
Objectives
- Learn the general structure of bacteria and the importance of plasmids.
- Learn micropipetting techniques.
- Conduct a bacteria transformation experiment using the heat shock method.
- Understand more about green fluorescent protein (GFP) and its uses.
- Understand applications of genetic transformation.
- Grow bacteria via plating and observe the results.
- Learn the sterile techniques required when working with bacteria.
Duration
2.5 hours
Time
9.30am or 2.30pm
Min Group Size
25 students per class
Max. Capacity
40 students per class
Age Group
15 – 18 years old
Remarks
Participants require a basic understanding of DNA and biology
